Proteintech, 26026-1-AP, C10orf88 Polyclonal antibody

Size: 20ul / 150ul The C10orf88 (26026-1-AP) by Proteintech is a Polyclonal antibody targeting C10orf88 in IHC, IF/ICC, IP, ELISA applications with reactivity to human, rat samples 26026-1-AP targets C10orf88 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive IP detected in: HEK-293 cells, SH-SY5Y cells Positive IHC detected in: rat pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information C10orf88 (Chromosome 10 Open Reading Frame 88) is a mitochondria-localized protein that is thought to be associated with protein-binding activity of mitochondria and plays a role in mitochondrial dysfunction and metabolic diseases. Studies suggest that C10orf88 may be involved in cellular metabolism, cell cycle regulation, and maintenance of mitochondrial homeostasis. Its activity is closely related to ATP synthesis and oxidative phosphorylation in mitochondria, and may be affected by a variety of metabolic regulators (e.g., metformin, AICAR) and cell cycle regulators (e.g., rosavitine). In addition, inhibitors of C10orf88 (e.g., oryzanamycin, rotenone) modulate its activity by affecting ATP synthase and electron transport chain complex I in mitochondria. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22971 Product name: Recombinant human C10orf88 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-445 aa of BC032645 Sequence: METRTEDGGLTRRPTLASSWDVAGGALTHSLLLTRAGLGPGDFDWEELLAPPAPGQDLVILKRNHNNKDENPCFLYLRCGPDGGEEIASIGILSSARNMEVYLGEEYCGTSRGKNVCTVLDDSEHEKIILYKKNLKLESSTHACKIKLLSFGERQCVFISKVVVHMRSVFANSSTSSPALGSRIDLDKVQTIMESMGSKLSPGAQQLMDMVRCQQRNCIPIGEQLQSVLGNSGYKHMIGLQSSSTLGTLNKSSSTPFPFRTGLTSGNVTENLQTYIDKSTQLPGGENSTKLDECKVMPQNHSFLENDLKNAMASFLPKKVSDNSNIPNSELLPFLQNLCSQVNHLHVGNKTECQENITKHGERILGVGMEEQSICSYLEKILSKNMELMEKKLMDYIDQRIHELQEHIDDKIALLLDLLQNPNSPPTGIPLRHYDSGERLSNGER Predict reactive species Full Name: chromosome 10 open reading frame 88 Calculated Molecular Weight: 445 aa, 49 kDa Observed Molecular Weight: 49 kDa GenBank Accession Number: BC032645 Gene Symbol: C10orf88 Gene ID (NCBI): 80007 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H8K7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.