Proteintech, 26071-1-AP, DUSP6 Polyclonal antibody

Size: 20ul / 150ul The DUSP6 (26071-1-AP) by Proteintech is a Polyclonal antibody targeting DUSP6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 26071-1-AP targets DUSP6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, PC-12 cells, mouse heart tissue Positive IP detected in: PC-12 cells Positive IHC detected in: mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, PC-12 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23474 Product name: Recombinant human DUSP6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 353-381 aa of BC003562 Sequence: CDNRVPAQQLYFTTPSNQNVYQVDSLQST Predict reactive species Full Name: dual specificity phosphatase 6 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC003562 Gene Symbol: DUSP6 Gene ID (NCBI): 1848 RRID: AB_3669521 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16828 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.