Proteintech, 26156-1-AP, IL-2 Polyclonal antibody

Size: 20ul / 150ul The IL-2 (26156-1-AP) by Proteintech is a Polyclonal antibody targeting IL-2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 26156-1-AP targets IL-2 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PMA, Ionomycin and Brefeldin A treated EL-4 cells Positive IHC detected in: human liver cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information What is the calculated molecular weight of IL2?19 kDaWhere is IL-2 produced?IL-2 is primarily produced and secreted by CD4+helper T cells in secondary lymphoid organs. CD8+T cells, some B cells, and dendritic cells also produce IL-2.What allows the protein to bind to its receptor?The N-terminal 20 amino acids are essential for protein-receptor interaction.What are the post-translational modifications of IL-2?IL-2 may present glycosylation, yet the function of this modification has not been confirmed.What is the role of IL-2?IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion.What activates the IL-2 pathway?Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway.How does IL-2 expression change during an immune response?During an immune response, CD4+and CD8+T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells.How do the responses to strong and weak IL-2 signals differ?Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells.What negatively regulates IL-2?Immunoregulatory CD4+CD5+T cells in inhibit IL-2 production by helper T cells.(PMIDs: 22343569, 24376444, 15473953) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24079 Product name: Recombinant mouse IL-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 21-169 aa of NM-008366 Sequence: APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ Predict reactive species Full Name: interleukin 2 Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 19 kDa GenBank Accession Number: NM-008366 Gene Symbol: IL-2 Gene ID (NCBI): 16183 RRID: AB_2880407 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04351 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.