Proteintech, 26229-1-AP, DNAJC25 Polyclonal antibody

Size: 20ul / 150ul The DNAJC25 (26229-1-AP) by Proteintech is a Polyclonal antibody targeting DNAJC25 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 26229-1-AP targets DNAJC25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, human placenta tissue, mouse liver tisue, rat liver tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DnaJ heat shock protein family (Hsp40) member C25 (DNAJC25), which belongs to the HSP40 subfamily C, is localized to the cytoplasm. DNAJC25 had a markedly high expression level in liver tissues and was significantly downregulated in liver cancer tissues compared with the adjacent normal tissues (PMID: 23205125). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23760 Product name: Recombinant human DNAJC25 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-360 aa of BC048318 Sequence: MGAPLLSPGWGAGAAGRRWWMLLAPLLPALLLVRPAGALVEGLYCGTRDCYEVLGVSRSAGKAEIARAYRQLARRYHPDRYRPQPGDEGPGRTPQSAEEAFLLVATAYETLKDEETRKDYDYMLDHPEEYYSHYYHYYSRRLAPKVDVRVVILVSVCAISVFQFFSWWNSYNKAISYLATVPKYRIQATEIAKQQGLLKKAKEKGKNKKSKEEIRDEEENIIKNIIKSKIDIKGGYQKPQICDLLLFQIILAPFHLCSYIVWYCRWIYNFNIKGKEYGEEERLYIIRKSMKMSKSQFDSLEDHQKETFLKRELWIKENYEVYKQEQEEELKKKLANDPRWKRYRRWMKNEGPGRLTFVDD Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily C , member 25 Observed Molecular Weight: 36-42 kDa GenBank Accession Number: BC048318 Gene Symbol: DNAJC25 Gene ID (NCBI): 548645 RRID: AB_3085849 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H1X3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.