Proteintech, 26707-1-AP, USP54 Polyclonal antibody

Size: 20ul / 150ul The USP54 (26707-1-AP) by Proteintech is a Polyclonal antibody targeting USP54 in WB, IHC, ELISA applications with reactivity to human samples 26707-1-AP targets USP54 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LNCaP cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information USP54 is a ubiquitin-specific protease in the deubiquitinating enzyme (DUB) family that promotes the malignant progression of many cancers. As a deubiquitinating enzyme, USP54 maintains the stability of PLK4 protein through deubiquitination, which promotes centrosome amplification and GC progression. USP54 is also highly expressed in colorectal cancers, and is associated with proliferation, invasion and migration of colorectal cancer. In addition, USP54 plays a key role in the transformation of hormone-sensitive prostate cancer (HSPC) into chemoresistant prostate cancer (CRPC), and may serve as a potential therapeutic target in CRPC. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25080 Product name: Recombinant human USP54 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 377-725 aa of BC130633 Sequence: DSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQHSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW Predict reactive species Full Name: ubiquitin specific peptidase 54 Observed Molecular Weight: 180 kDa GenBank Accession Number: BC130633 Gene Symbol: USP54 Gene ID (NCBI): 159195 RRID: AB_3669564 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q70EL1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.