Proteintech, 26721-1-AP, DNAJC3 Polyclonal antibody

Size: 20ul / 150ul The DNAJC3 (26721-1-AP) by Proteintech is a Polyclonal antibody targeting DNAJC3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 26721-1-AP targets DNAJC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse testis tissue Positive IHC detected in: human colon cancer tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24966 Product name: Recombinant human DNAJC3 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 32-380 aa of BC047936 Sequence: GVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGL Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily C, member 3 Observed Molecular Weight: 58 kDa GenBank Accession Number: BC047936 Gene Symbol: DNAJC3 Gene ID (NCBI): 5611 RRID: AB_2880613 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13217 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.