Proteintech, 26933-1-AP, CCNK Polyclonal antibody

Size: 20ul / 150ul The CCNK (26933-1-AP) by Proteintech is a Polyclonal antibody targeting CCNK in WB, IHC, ELISA applications with reactivity to human samples 26933-1-AP targets CCNK in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:1000 Background Information Cyclin-K (CCNK) is also named as CPR4 and belongs to the cyclin family. CCNK is a regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases (PMID:10574912). It plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A) (PMID:9632813). The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes (PMID:22012619). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25556 Product name: Recombinant human CCNK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC015935 Sequence: MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWACRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWGGQPG Predict reactive species Full Name: cyclin K Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC015935 Gene Symbol: CCNK Gene ID (NCBI): 8812 RRID: AB_2935464 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75909 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.