Proteintech, 26936-1-AP, SPTB Polyclonal antibody

Size: 20ul / 150ul The SPTB (26936-1-AP) by Proteintech is a Polyclonal antibody targeting SPTB in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 26936-1-AP targets SPTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, mouse heart tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Beta I spectrin also known as SPTB, SPTB1, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTB have been associated with Elliptocytosis 3, spherocytosis type 2, and neonatal hemolytic anemia(PMID: 8018926, 8102379, 9005995). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25614 Product name: Recombinant human SPTB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1800-1900 aa of BC136284 Sequence: HRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLV Predict reactive species Full Name: spectrin, beta, erythrocytic Observed Molecular Weight: 246 kDa GenBank Accession Number: BC136284 Gene Symbol: SPTB Gene ID (NCBI): 6710 RRID: AB_3669575 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11277 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.