Proteintech, 27168-1-AP, RANBP3 Polyclonal antibody

Size: 20ul / 150ul The RANBP3 (27168-1-AP) by Proteintech is a Polyclonal antibody targeting RANBP3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 27168-1-AP targets RANBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells Positive IHC detected in: mouse testis tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RANBP3 (Ran-binding protein 3) is also named as Rab escort protein 1 (REP-1). RanBP3 is a RanGTP‐binding protein that has been reported to enhance nuclear export of active β‐catenin, independent of its role as a CRM1‐associated nuclear export cofactorl (PMID: 9637251). RanBP3 has been reported to be a crucial downstream molecule of MAPK-ERK and PI3K-AKT signal pathways (PMID: 18331709). RanBP3 is a tumor suppressor in CRC and is associated with patient survival (PMID: 36270974). RanBP3 has been reported as a potential regulator in melanoma biology, hepatocellular carcinoma, prostate cancer, and ovarian cancer, and RanBP3 is highly expressed in CML Cells (PMID: 34504783). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26000 Product name: Recombinant human RANBP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 33-176 aa of BC004349 Sequence: SPEGGEDSDREDGNYCPPVKRERTSSLTQFPPSQSEERSSGFRLKPPTLIHGQAPSAGLPSQKPKEQQRSVLRPAVLQAPQPKALSQTVPSSGTNGVSLPADCTGAVPAASPDTAAWRSPSEAADEVCALEEKEPQKNESSNAS Predict reactive species Full Name: RAN binding protein 3 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC004349 Gene Symbol: RANBP3 Gene ID (NCBI): 8498 RRID: AB_3669586 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H6Z4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.