Proteintech, 27176-1-AP, ST14 Polyclonal antibody

Size: 20ul / 150ul The ST14 (27176-1-AP) by Proteintech is a Polyclonal antibody targeting ST14 in WB, IHC, ELISA applications with reactivity to human samples 27176-1-AP targets ST14 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LNCaP cells, MCF-7 cells Positive IHC detected in: human colon tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26007 Product name: Recombinant human ST14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 301-539 aa of BC030532 Sequence: YNLTFHSSQNVLLITLITNTERRHPGFEATFFQLPRMSSCGGRLRKAQGTFNSPYYPGHYPPNIDCTWNIEVPNNQHVKVRFKFFYLLEPGVPAGTCPKDYVEINGEKYCGERSQFVVTSNSNKITVRFHSDQSYTDTGFLAEYLSYDSSDPCPGQFTCRTGRCIRKELRCDGWADCTDHSDELNCSCDAGHQFTCKNKFCKPLFWVCDSVNDCGDNSDEQGCSCPAQTFRCSNGKCLS Predict reactive species Full Name: suppression of tumorigenicity 14 (colon carcinoma) Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC030532 Gene Symbol: ST14 Gene ID (NCBI): 6768 RRID: AB_2880788 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y5Y6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.