Proteintech, 27207-1-AP, BDH2 Polyclonal antibody

Size: 20ul / 150ul The BDH2 (27207-1-AP) by Proteintech is a Polyclonal antibody targeting BDH2 in WB, IHC, ELISA applications with reactivity to human samples 27207-1-AP targets BDH2 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information Type 2-hydroxybutyrate dehydrogenase (BDH2) is a short-chain dehydrogenase/reductase family member involved in several biological and pathological processes, including the utilization of cytosolic ketone bodies, immunocyte regulation and tumor progression. As a member of a gene family involved in ketone metabolism, BDH2 upregulated the level of β-HB in liver cells as well as the level of H3 histone acetylation (PMID: 25762501). Downregulation of BDH2 during infection is due to activation of hepcidin and intracellular iron retention, indicating an important role for BDH2 in iron-limiting innate immunity and host defense (PMID: 31333791, 32344823). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24934 Product name: Recombinant human BDH2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC095414 Sequence: MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFANEVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGRFATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL Predict reactive species Full Name: 3-hydroxybutyrate dehydrogenase, type 2 Observed Molecular Weight: 27 kDa GenBank Accession Number: BC095414 Gene Symbol: BDH2 Gene ID (NCBI): 56898 RRID: AB_2880800 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BUT1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.