Proteintech, 27279-1-AP, KCTD10 Polyclonal antibody

Size: 20ul / 150ul The KCTD10 (27279-1-AP) by Proteintech is a Polyclonal antibody targeting KCTD10 in WB, IHC, IP, ELISA applications with reactivity to human samples 27279-1-AP targets KCTD10 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HuH-7 cells, Jurkat cells, K-562 cells, MCF-7 cells, U2OS cells Positive IP detected in: HuH-7 cells Positive IHC detected in: human breast cancer tissue, human intrahepatic cholangiocarcinoma tissue, human prostate hyperplasia tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25971 Product name: Recombinant human KCTD10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 253-313 aa of BC040062 Sequence: TLNILLYEAQDGRGPDNALLEATGGAAGRSHHLDEDEERERIERVRRIHIKRPDDRAHLHQ Predict reactive species Full Name: potassium channel tetramerisation domain containing 10 Calculated Molecular Weight: 313 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC040062 Gene Symbol: KCTD10 Gene ID (NCBI): 83892 RRID: AB_2880827 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H3F6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.