Proteintech, 27293-1-AP, Chordin Polyclonal antibody

Size: 20ul / 150ul The Chordin (27293-1-AP) by Proteintech is a Polyclonal antibody targeting Chordin in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 27293-1-AP targets Chordin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 3T3-L1 cells Positive IHC detected in: human liver cancer tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26001 Product name: Recombinant human CHRD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-350 aa of BC112345 Sequence: AGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQA Predict reactive species Full Name: chordin Calculated Molecular Weight: 955 aa, 102 kDa Observed Molecular Weight: 102 kDa GenBank Accession Number: BC112345 Gene Symbol: Chordin Gene ID (NCBI): 8646 RRID: AB_2880832 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H2X0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.