Proteintech, 27751-1-AP, APOB Polyclonal antibody

Size: 20ul / 150ul The APOB (27751-1-AP) by Proteintech is a Polyclonal antibody targeting APOB in WB, IHC, ELISA applications with reactivity to human, mouse samples 27751-1-AP targets APOB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue, HepG2 cells Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. Unlike humans and rabbits, rats and mice secrete both apoB-100- and apoB-48-containing lipoproteins from the liver as a result of the hepatic expression of APOBEC-1 (PMID: 9633945). This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26773 Product name: Recombinant human APOB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 23-220 aa of NM_000384 Sequence: MAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRT Predict reactive species Full Name: apolipoprotein B (including Ag(x) antigen) Calculated Molecular Weight: 516 kDa Observed Molecular Weight: 150-250 kDa, 400-520 kDa GenBank Accession Number: NM_000384 Gene Symbol: APOB Gene ID (NCBI): 338 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04114 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.