Proteintech, 27861-1-AP, GDPD1 Polyclonal antibody

Size: 20ul / 150ul The GDPD1 (27861-1-AP) by Proteintech is a Polyclonal antibody targeting GDPD1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 27861-1-AP targets GDPD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, mouse ovary tissue, rat testis tissue Positive IHC detected in: mouse testis tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Glycerophosphodiester phosphodiesterase domain-containing protein 1 (GDPD1) is an enzyme that hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. GDPD1 shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylethanolamine (lyso-PE) and lysophosphatidylcholine (lyso-PC). GDPD1 may be involved in bioactive N-acylethanolamine biosynthesis from both N-acyl-lysoplasmenylethanolamin (N-acyl-lysoPlsEt) and N-acyl-lysophosphatidylethanolamin (N-acyl-lysoPE) (PMID:25596343, PMID:27637550). GDPD1 is widely expressed with a high expression level in testis. GDPD1 is a prognostic marker in Liver hepatocellular carcinoma. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27371 Product name: Recombinant human GDPD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 30-195 aa of BC034432 Sequence: LHQRKKQRFLSKHISHRGGAGENLENTMAAFQHAVKIGTDMLELDCHITKDEQVVVSHDENLKRATGVNVNISDLKYCELPPYLGKLDVSFQRACQCEGKDNRIPLLKEVFEAFPNTPINIDIKVNNNVLIKKVSELVKRYNREHLTVWGNANYEIVEKCYKENSD Predict reactive species Full Name: glycerophosphodiester phosphodiesterase domain containing 1 Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 33-36 kDa GenBank Accession Number: BC034432 Gene Symbol: GDPD1 Gene ID (NCBI): 284161 RRID: AB_2880996 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N9F7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.