Proteintech, 27874-1-AP, APBB1 Polyclonal antibody

Size: 20ul / 150ul The APBB1 (27874-1-AP) by Proteintech is a Polyclonal antibody targeting APBB1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 27874-1-AP targets APBB1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, mouse brain tissue, SH-SY5Y cells, U-87 MG cells, U2OS cells, rat brain tissue Positive IP detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Adaptor protein FE65 (APBB1) specifically binds to the intracellular tail of the type I transmembrane protein, β-amyloid precursor protein (APP). FE65 is a multimodular adaptor protein, consisting of three major protein-protein interaction domains, a WW domain and two phosphotyrosine interaction domains (PID). The interaction between FE65 and APP mainly takes place between the C-terminal PID (PID2) and the APP intracellular domain (AICD) (PMID: 15647266). There is novel evidence that the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis. The cleavage region locates immediately after an acidic residue cluster but before the three major protein-protein binding domains (PMID: 15647266). FE65 is predominantly expressed in central nervous system neurons, and its expression is regulated during development and aging, with high levels corresponding to the timing of neural tissue formation and high neuronal activity (PMID: 7867517, PMID: 10561691, PMID: 14689444). p65FE65 (65kDa) is endoproteolytically cleaved from p97FE65 (97 kDa), the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis (PMID: 15647266). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27434 Product name: Recombinant human APBB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 43-268 aa of BC010854 Sequence: TAVGPKDLRSAMGEGGGPEPGPANAKWLKEGQNQLRRAATAHRDQNRNVTLTLAEEASQEPEMAPLGPKGLIHLYSELELSAHNAANRGLRGPGLIISTQEQGPDEGEEKAAGEAEEEEEDDDDEEEEEDLSSPPGLPEPLESVEAPPRPQALTDGPREHSKSASLLFGMRNSAASDEDSSWATLSQGSPSYGSPEDTDSFWNPNAFETDSDLPAGWMRVQDTSGT Predict reactive species Full Name: amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) Calculated Molecular Weight: 708 aa, 77 kDa Observed Molecular Weight: 65 kDa, 97 kDa GenBank Accession Number: BC010854 Gene Symbol: APBB1 Gene ID (NCBI): 322 RRID: AB_3669628 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00213 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.