Proteintech, 27899-1-AP, TRIB1 Polyclonal antibody

Size: 20ul / 150ul The TRIB1 (27899-1-AP) by Proteintech is a Polyclonal antibody targeting TRIB1 in WB, IHC, ELISA applications with reactivity to human samples 27899-1-AP targets TRIB1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MDA-MB-231 cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TRIB1, a pseudokinase encoded by the tribbles homolog 1 gene, regulates hepatic lipogenesis and lipid metabolism by modulating C/EBPα degradation, thereby influencing plasma lipid profiles and atherosclerotic risk. It is expressed in macrophages and vascular smooth muscle cells, linking metabolic and inflammatory pathways in cardiometabolic disorders (PMID: 26517927, PMID: 40331689). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27541 Product name: Recombinant human TRIB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC063292 Sequence: MRVGPVRSAMSGASQPRGPALLFPATRGVPAKRLLDADDAAAVAAKCPRLSECSSPPDYLSPPGSPCSPQPPPAAPGAGGGSGSAPGPSRIADYLLLPLAEREHVSRALCIHTGRELRCKVFPIKHYQDKIRPYIQLPSHSNITGIVEVI Predict reactive species Full Name: tribbles homolog 1 (Drosophila) Calculated Molecular Weight: 372 aa, 41 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: BC063292 Gene Symbol: TRIB1 Gene ID (NCBI): 10221 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96RU8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.