Proteintech, 28136-1-AP, Caspase 14 Polyclonal antibody

Size: 20ul / 150ul The Caspase 14 (28136-1-AP) by Proteintech is a Polyclonal antibody targeting Caspase 14 in WB, ELISA applications with reactivity to human, mouse, rat samples 28136-1-AP targets Caspase 14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skin tissue, rat skin tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Caspase14 belongs to the caspase family of cysteinyl aspartate-specific proteinases that frequently play a central role in apoptosis. Caspase14 exist as inactive proenzymes that undergo proteolytic processing to produce large and small subunits (p20 and p10) at Ile152/Lys153 residues, which is different to other caspases (Asp residue). Caspase14 plays a role in protein maturation of filaggrin, and possibly in DNA repair. Overexpression of Caspase14 has been detected in several of epithelial malignancies, indicating that Caspase14 could play a role in carcinogenesis and disease progression of cancers. Caspase14 can binds the apoptosis-inducing factor (AIF) indicating that caspase14 may be an anti-apoptotic protein. In the LADC specimens, two bands can be detected with MW of 35 kDa and 37 kDa which represent Caspase14 and the phosphorylated Caspase14(pCasp-14) respectively. (PMID:21567094, 12200134, 10203698, 17515931, 16061209). This antibody can recognise all the forms of Caspase14. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27242 Product name: Recombinant human CASP14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-242 aa of BC069541 Sequence: MSNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQFQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDPGETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRHDQKGSCFIQTLVDVFTKRKGHILELLTEVTRRMAEAELVQEGKARKTNPEIQSTLRKRLYLQ Predict reactive species Full Name: caspase 14, apoptosis-related cysteine peptidase Calculated Molecular Weight: 242 aa, 28 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC069541 Gene Symbol: CASP14 Gene ID (NCBI): 23581 RRID: AB_3086031 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31944 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.