Proteintech, 28164-1-AP, IRE1/ERN1 Polyclonal antibody

Size: 20ul / 150ul The IRE1/ERN1 (28164-1-AP) by Proteintech is a Polyclonal antibody targeting IRE1/ERN1 in WB, ELISA applications with reactivity to human samples 28164-1-AP targets IRE1/ERN1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27375 Product name: Recombinant human IRE1; ERN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 233-444 aa of BC130405 Sequence: QREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDM Predict reactive species Full Name: endoplasmic reticulum to nucleus signaling 1 Calculated Molecular Weight: 977 aa, 110 kDa Observed Molecular Weight: 130 kDa GenBank Accession Number: BC130405 Gene Symbol: IRE1 Gene ID (NCBI): 2081 RRID: AB_3086033 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75460 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.