Proteintech, 28217-1-AP, RAI2 Polyclonal antibody

Size: 20ul / 150ul The RAI2 (28217-1-AP) by Proteintech is a Polyclonal antibody targeting RAI2 in WB, IF/ICC, ELISA applications with reactivity to human samples 28217-1-AP targets RAI2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, IMR-32 cells, SK-N-SH cells, TT cells Positive IF/ICC detected in: SW480 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Retinoic acid-induced 2 (RAI2) was initially described as a retinoic acid-inducible gene, which has been assumed to be involved in development. RAI2 was initially related to cell differentiation and induced by retinoic acid and has been identified as an emerging tumor suppressor in breast cancer and colorectal cancer. RAI2 is frequently methylated in human CRC, and the expression of RAI2 is regulated by promoter region methylation (PMID: 29796120). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28253 Product name: Recombinant human RAI2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 282-512 aa of BC027937 Sequence: PLEKDELKPFDILQPKEYFQLSRHTVIKMGSENEALDLSMKSVPWLKAGEVSPPIFQEDAPLDLSVAAHRKSEPPPETLYDSGASVDSSGHTVMEKLPSGMEISFAPATSHEAPAMMDSHISSSDAATEMLSQPNHPSGEVKAENNIEMVGESQAAKVIVSVEDAVPTIFCGKIKGLSGVSTKNFSFKREDSVLQGYDINSQGEESMGNAEPLRKPIKNRSIKLKKVNSQE Predict reactive species Full Name: retinoic acid induced 2 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC027937 Gene Symbol: RAI2 Gene ID (NCBI): 10742 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y5P3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.