Proteintech, 28219-1-AP, PCSK1 Polyclonal antibody

Size: 20ul / 150ul The PCSK1 (28219-1-AP) by Proteintech is a Polyclonal antibody targeting PCSK1 in WB, IHC, ELISA applications with reactivity to Human, Mouse, rat samples 28219-1-AP targets PCSK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples. Tested Applications Positive WB detected in: BxPC-3 cells, HeLa cells Positive IHC detected in: human pancreas tissue, mouse pancreas tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein. Specification Tested Reactivity: Human, Mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28203 Product name: Recombinant human PCSK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 406-585 aa of BC130295 Sequence: DMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIV Predict reactive species Full Name: proprotein convertase subtilisin/kexin type 1 Calculated Molecular Weight: 753 aa, 84 kDa Observed Molecular Weight: 66 kDa GenBank Accession Number: BC130295 Gene Symbol: PCSK1 Gene ID (NCBI): 5122 RRID: AB_2881093 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29120 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.