Proteintech, 28232-1-AP, GPR4 Polyclonal antibody

Size: 20ul / 150ul The GPR4 (28232-1-AP) by Proteintech is a Polyclonal antibody targeting GPR4 in IHC, ELISA applications with reactivity to human, mouse samples 28232-1-AP targets GPR4 in IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IHC detected in: mouse lung tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28273 Product name: Recombinant human GPR4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 203-362 aa of BC067535 Sequence: GILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ Predict reactive species Full Name: G protein-coupled receptor 4 Calculated Molecular Weight: 362 aa, 41 kDa GenBank Accession Number: BC067535 Gene Symbol: GPR4 Gene ID (NCBI): 2828 RRID: AB_3086039 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P46093 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.