Proteintech, 28486-1-AP, BRD4 Polyclonal antibody

Size: 20ul / 150ul The BRD4 (28486-1-AP) by Proteintech is a Polyclonal antibody targeting BRD4 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 28486-1-AP targets BRD4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, A549 cells, Jurkat cells, MCF-7 cells, Ramos cells, MDA-MB-231 cells, T-47D cells Positive IP detected in: A549 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. This antibody detects the small and the long isoform of BRD4, the molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: bromodomain containing 4 Calculated Molecular Weight: 1362 aa, 152 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC035266 Gene Symbol: BRD4 Gene ID (NCBI): 23476 RRID: AB_2918170 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60885 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.