Proteintech, 28525-1-AP, NMDAR2A/GRIN2A Polyclonal antibody

Size: 20ul / 150ul The NMDAR2A/GRIN2A (28525-1-AP) by Proteintech is a Polyclonal antibody targeting NMDAR2A/GRIN2A in WB, IP, IHC, ELISA applications with reactivity to mouse, rat samples 28525-1-AP targets NMDAR2A/GRIN2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GRIN2A (glutamate ionotropic receptor NMDA type subunit 2A), also known as NMDAR2A. And its molecular weight is 165 kDa. GRIN2A is located in cell projection, dendritic spine, cell membrane, synapse, postsynaptic cell membrae, cytolamic vesicle membrane, which is expressed in many tissues, highest expression in brain and heart. This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without cognitive disability. Alternative splicing results in multiple transcript variants. Specification Tested Reactivity: mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29101 Product name: Recombinant human NMDAR2A/GRIN2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 933-1127 aa of NM_000833 Sequence: DMVSDKGNLMYSDNRSFQGKESIFGDNMNELQTFVANRQKDNLNNYVFQGQHPLTLNESNPNTVEVAVSTESKANSRPRQLWKKSVDSIRQDSLSQNPVSQRDEATAENRTHSLKSPRYLPEEMAHSDISETSNRATCHREPDNSKNHKTKDNFKRSVASKYPKDCSEVERTYLKTKSSSPRDKIYTIDGEKEPG Predict reactive species Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 2A Calculated Molecular Weight: 165 kDa Observed Molecular Weight: 160-180 kDa GenBank Accession Number: NM_000833 Gene Symbol: GRIN2A Gene ID (NCBI): 2903 RRID: AB_2881163 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12879 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.