Proteintech, 28757-1-AP, Phospho-FOXO1 (Ser319) Polyclonal antibody

Size: 20ul / 100ul The Phospho-FOXO1 (Ser319) (28757-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-FOXO1 (Ser319) in WB, ELISA applications with reactivity to Human samples 28757-1-AP targets Phospho-FOXO1 (Ser319) in WB, IF, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: MCF-7 cells, Cobalt Chloride treated MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593) Specification Tested Reactivity: Human Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: forkhead box O1 Calculated Molecular Weight: 655 aa, 70 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC021981 Gene Symbol: FOXO1 Gene ID (NCBI): 2308 RRID: AB_3669669 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12778 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.