Proteintech, 28790-1-AP, Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal antibody

Size: 20ul / 100ul The Phospho-ATF2 (Thr71)/ATF7 (Thr53) (28790-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-ATF2 (Thr71)/ATF7 (Thr53) in WB, ELISA applications with reactivity to Human, Mouse samples 28790-1-AP targets Phospho-ATF2 (Thr71)/ATF7 (Thr53) in WB, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: Anisomycin treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Background Information ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53. Specification Tested Reactivity: Human, Mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: activating transcription factor 2 Calculated Molecular Weight: 209 aa, 23 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC026175 Gene Symbol: ATF2 Gene ID (NCBI): 1386 RRID: AB_3085335 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15336 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.