Proteintech, 28805-1-AP, Phospho-Chk1 (Ser296) Polyclonal antibody

Size: 20ul / 100ul The Phospho-Chk1 (Ser296) (28805-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-Chk1 (Ser296) in WB, ELISA applications with reactivity to human, mouse, rat samples 28805-1-AP targets Phospho-Chk1 (Ser296) in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Calyculin A treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated (PMID:22941630) and ubiquitinated (PMID:19276361). Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: CHK1 checkpoint homolog (S. pombe) Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC004202 Gene Symbol: Chk1 Gene ID (NCBI): 1111 RRID: AB_2881215 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14757 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.