Proteintech, 28824-1-AP, LTBP2 Polyclonal antibody

Size: 20ul / 150ul The LTBP2 (28824-1-AP) by Proteintech is a Polyclonal antibody targeting LTBP2 in IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 28824-1-AP targets LTBP2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: U2OS cells Positive IHC detected in: mouse lung tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information LTBP2, an extracellular glycoprotein mapping to chromosome 14q24, belongs to a family of latent TGF-β binding proteins (LTBPs) that regulates a well-known growth factor, TGF-β. LTBP2 is an elastic fiber-associating protein whose function in elastogenesis is not clear, as a DANCEbinding Protein. (PMID: 17581631 )It may play an integral structural role in elastic-fiber architectural organization and/or assembly. LTBP2 associates covalently with small latent TGF-beta complex via Repeat B and Repeat C. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). [MIM:613086] LTBP2 is a role in the structural stability of ciliary zonules, and growth and development of lens.(PMID: 20617341) LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma (PMID: 20179738 ) . LTBP2 is a biomarker of heart failure. LTBP2 has a molecular weight of 260 kDa (PMID: 20878956), and even larger molecular weights of 290 and 310 kDa were detected in tissue extracts. (PMID: 8524260) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29737 Product name: Recombinant human LTBP2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 241-376 aa of BC078659 Sequence: ERSPNLRRSSAAGEGTLARAQPPAPQSPPAPQSPPAGTLSGLSQTHPSQQHVGLSRTVRLHPTATASSQLSSNALPPGPGLEQRDGTQQAVPLEHPSSPWGLNLTEKIKKIKIVFTPTICKQTCARGHCANSCERG Predict reactive species Full Name: latent transforming growth factor beta binding protein 2 Calculated Molecular Weight: 1821 aa, 195 kDa Observed Molecular Weight: 260 kDa GenBank Accession Number: BC078659 Gene Symbol: LTBP2 Gene ID (NCBI): 4053 RRID: AB_3669670 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14767 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.