Proteintech, 28835-1-AP, CELSR3 Polyclonal antibody

Size: 20ul / 150ul The CELSR3 (28835-1-AP) by Proteintech is a Polyclonal antibody targeting CELSR3 in IHC, IF-P, ELISA applications with reactivity to Human, Mouse samples 28835-1-AP targets CELSR3 in IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse cerebellum tissue Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Atypical cadherin Celsr3 plays critical roles during brain development, in collaboration with Fzd3. Celsr3 is required for postnatal cerebellar Purkinje cell maturation and synaptic plasticity, which has potential implications in neural disorders implicating the cerebellum. Specification Tested Reactivity: Human, Mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28746 Product name: Recombinant human CELSR3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2285-2540 aa of NM_001407 Sequence: MPGGSPGSAGLVRHLEEYAATLARNMELTYLNPMGLVTPNIMLSIDRMEHPSSPRGARRYPRYHSNLFRGQDAWDPHTHVLLPSQSPRPSPSEVLPTSSSIENSTTSSVVPPPAPPEPEPGISIIILLVYRTLGGLLPAQFQAERRGARLPQNPVMNSPVVSVAVFHGRNFLRGILESPISLEFRLLQTANRSKAICVQWDPPGLAEQHGVWTARDCELVHRNGSHARCRCSRTGTFGVLMDASPRERLEGDLELLA Predict reactive species Full Name: cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila) Calculated Molecular Weight: 358 kDa GenBank Accession Number: NM_001407 Gene Symbol: CELSR3 Gene ID (NCBI): 1951 RRID: AB_3086089 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NYQ7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.