Proteintech, 28853-1-AP, Phospho-Beta Catenin (Ser675) Polyclonal antibody

Size: 20ul / 100ul The Phospho-Beta Catenin (Ser675) (28853-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-Beta Catenin (Ser675) in WB, IF/ICC, ELISA applications with reactivity to Human samples 28853-1-AP targets Phospho-Beta Catenin (Ser675) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: λ phosphatase treated HT-29 cells Positive IF/ICC detected in: λ phosphatase treated MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Beta Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. Beta catenin is a core component of the cadherin protein complex, whose stabilization is essential for the activation of Wnt/β-catenin signaling. Phosphorylation of Ser675 in the C-terminus of at beta catenin promotesmits nuclear accumulation and transcriptional activity, resulting in increased activation of the Wnt/β-catenin signaling pathway(PMID: 25980439). In addition, Ser675 phosphorylation promotes beta catenin interaction with the transcriptional coactivator CREB-binding protein in the nucleus(PMID: 16476742). Specification Tested Reactivity: Human Cited Reactivity: human, mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: catenin (cadherin-associated protein), beta 1, 88kDa Calculated Molecular Weight: 781 aa, 86 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC058926 Gene Symbol: Beta Catenin Gene ID (NCBI): 1499 ENSEMBL Gene ID: ENSG00000168036 RRID: AB_2923583 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35222 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.