Proteintech, 28855-1-AP, Phospho-GYS (Ser641) Polyclonal antibody

Size: 20ul / 100ul The Phospho-GYS (Ser641) (28855-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-GYS (Ser641) in WB, IF/ICC, ELISA applications with reactivity to human samples 28855-1-AP targets Phospho-GYS (Ser641) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: λ phosphatase treated PC-3 cells Positive IF/ICC detected in: λ phosphatase treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in the liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites, and insulin stimulates the dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity, which will decrease phosphorylation of GS and increase glycogen synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID:22232606) Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: glycogen synthase 1 (muscle) Calculated Molecular Weight: 84 kDa Observed Molecular Weight: 84 kDa GenBank Accession Number: BC007688 Gene Symbol: GYS1 Gene ID (NCBI): 2997 RRID: AB_2923584 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13807 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.