Proteintech, 28945-1-AP, Phospho-STAT3 (Ser727) Polyclonal antibody

Size: 20ul / 100ul The Phospho-STAT3 (Ser727) (28945-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-STAT3 (Ser727) in WB, ELISA applications with reactivity to human samples 28945-1-AP targets Phospho-STAT3 (Ser727) in WB, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, λ phosphatase treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 3 (acute-phase response factor) Calculated Molecular Weight: 770 aa, 88 kDa Observed Molecular Weight: 92-100 kDa GenBank Accession Number: BC000627 Gene Symbol: STAT3 Gene ID (NCBI): 6774 RRID: AB_3206262 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P40763 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.