Proteintech, 29027-1-AP, Phospho-MOBKL1B (Thr12) Polyclonal antibody

Size: 20ul / 100ul The Phospho-MOBKL1B (Thr12) (29027-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-MOBKL1B (Thr12) in WB, IF/ICC, ELISA applications with reactivity to human, rat samples 29027-1-AP targets Phospho-MOBKL1B (Thr12) in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: PC-12 cells, λ phosphatase treated PC-12 cells Positive IF/ICC detected in: λ phosphatase treated U2OS cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708). Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: MOB1, Mps One Binder kinase activator-like 1B (yeast) Calculated Molecular Weight: 216 aa, 25 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC003398 Gene Symbol: MOBKL1B Gene ID (NCBI): 55233 RRID: AB_2923589 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H8S9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.