Proteintech, 29117-1-AP, Phospho-RICTOR (Thr1135) Polyclonal antibody

Size: 20ul / 100ul The Phospho-RICTOR (Thr1135) (29117-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-RICTOR (Thr1135) in WB, ELISA applications with reactivity to Human samples 29117-1-AP targets Phospho-RICTOR (Thr1135) in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: PDGF treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Rictor is the core component of the mammalian target of rapamycin complex 2 (mTORC2), and loss of this protein leads to an immunodeficiency. RICTOR is regulated by phosphorylation at threonine 1135 (Thr1135) by S6K, downstream of PI3K, which inactivates mTORC2 and therefore inhibits AKT phosphorylation at Ser473. (PMID: 26356562, PMID: 28821013) Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: rapamycin-insensitive companion of mTOR Calculated Molecular Weight: 192 kDa Observed Molecular Weight: 192-200 kDa GenBank Accession Number: BC029608 Gene Symbol: RICTOR Gene ID (NCBI): 253260 RRID: AB_3085343 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6R327 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.