Proteintech, 29119-1-AP, Phospho-ACC1 (Ser79) Polyclonal antibody

Size: 20ul / 100ul The Phospho-ACC1 (Ser79) (29119-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-ACC1 (Ser79) in WB, ELISA applications with reactivity to Human, mouse, rat samples 29119-1-AP targets Phospho-ACC1 (Ser79) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: λ phosphatase treated SH-SY5Y cells, λ phosphatase treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information ACC1 represents a key enzyme, as it is highly regulated by phosphorylation and allosteric regulation, providing a rapid adaptation to new micro-environmental conditions. AMPK phosphorylates acetyl CoA carboxylase (ACC), a rate-controlling step in the conversion of acetyl-CoA to malonyl CoA. This phosphorylation inhibits the activity of ACC, which results in decreased malonyl CoA levels. Additionally, two isoforms of ACC encoded by two different genes in mammalian cells have been described, ACC1 and ACC2. ACC1 is highly enriched in lipogenic tissues (liver and adipose), while ACC2 is mainly expressed in oxidative tissues (heart, skeletal muscle and liver). (PMID: 29056512, PMID: 16054041, PMID: 30816537) Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: acetyl-Coenzyme A carboxylase alpha Calculated Molecular Weight: 2383 aa, 275 kDa Observed Molecular Weight: 250 kDa GenBank Accession Number: BC137287 Gene Symbol: Acetyl-CoA Carboxylase 1 Gene ID (NCBI): 31 RRID: AB_2918238 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13085 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.