Proteintech, 29145-1-AP, Phospho-MARCKS (Ser167/170) Polyclonal antibody

Size: 20ul / 100ul The Phospho-MARCKS (Ser167/170) (29145-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-MARCKS (Ser167/170) in WB, IF/ICC, ELISA applications with reactivity to Human samples 29145-1-AP targets Phospho-MARCKS (Ser167/170) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: λ phosphatase treated HeLa cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The Myristoylated Alanine Rich C-Kinase Substrate (MARCKS) is a ubiquitous, highly conserved protein among vertebrates, which is essential for postnatal survival, and has been widely studied for its functions in the brain and nervous system. Being highly expressed in nervous tissue, particularly during early development but persisting in the adult, it plays numerous roles related to brain growth, neuronal migration, neurite outgrowth, neurotransmitter release, and synaptic plasticity. Protein kinase C (PKC) phosphorylates MARCKS, which converts MARCKS from a membrane-bound protein to a cytoplasmic protein. The phosphorylation site of MARCKS protein is called the effector domain (ED). Its structure is highly conserved. It can be combined with cell membrane, PKC, calcium/calmodulin-dependent kineses (CaMK) and F-actin. Studies have shown that increased membrane-bound, non-phosphorylated MARCKS might be conducive to the stabilization of synaptic morphology. Phosphorylated MARCKS protein (P-MARCKS) can regulate the stability of actin network and alter the synaptic structure. (PMID: 30655546, PMID: 30155805) Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myristoylated alanine-rich protein kinase C substrate Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 80 kDa GenBank Accession Number: BC089040 Gene Symbol: MARCKS Gene ID (NCBI): 4082 RRID: AB_2923590 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29966 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.