Proteintech, 29304-1-AP, PAFAH Polyclonal antibody

Size: 20ul / 150ul The PAFAH (29304-1-AP) by Proteintech is a Polyclonal antibody targeting PAFAH in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 29304-1-AP targets PAFAH in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: RAW 264.7 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Platelet-activating factor acetylhydrolase (PAFAH) Ib consists of homo- or heterodimers of two PLA2 subunits α1 and α2, and a dimer of a third subunit, LIS1. PAFAH Ib was first discovered by its ability to hydrolyze the extracellular signaling lipid, PAF, which plays a role in platelet activation and inflammation. LIS1, the causative agent of the fatal brain disorder Miller-Dieker lissencephaly, functions independently of the α subunits to regulate the activity of cytoplasmic dynein. LIS1 is a highly conserved protein involved in dynein-mediated processes including nuclear and neuronal migration, centrosomal function, and mitotic spindle orientation. (PMID: 20624900, PMID: 23262398) Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30947 Product name: Recombinant human PLA2G7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 175-290 aa of BC038452 Sequence: AAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFR Predict reactive species Full Name: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 29-30 kDa, 45-55 kDa GenBank Accession Number: BC038452 Gene Symbol: PLA2G7 Gene ID (NCBI): 7941 RRID: AB_2918276 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13093 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.