Proteintech, 29618-1-AP, HTRA1 Polyclonal antibody

Size: 20ul / 150ul The HTRA1 (29618-1-AP) by Proteintech is a Polyclonal antibody targeting HTRA1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 29618-1-AP targets HTRA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, human placenta tissue Positive IP detected in: human placenta tissue Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 51 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31194 Product name: Recombinant human HTRA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 358-480 aa of NM_002775 Sequence: DKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDP Predict reactive species Full Name: HtrA serine peptidase 1 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: NM_002775 Gene Symbol: HTRA1 Gene ID (NCBI): 5654 RRID: AB_3086146 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92743 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.