Proteintech, 29715-1-AP, Phospho-Cofilin (Ser3) Polyclonal antibody

Size: 20ul / 100ul The Phospho-Cofilin (Ser3) (29715-1-AP) by Proteintech is a Polyclonal antibody targeting Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications with reactivity to Human samples 29715-1-AP targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: λ phosphatase treated HeLa cells, HeLa cells Positive IF/ICC detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm. Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cofilin 1 (non-muscle) Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC012318 Gene Symbol: Cofilin Gene ID (NCBI): 1072 RRID: AB_2935421 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P23528 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.