Proteintech, 29853-1-AP, MIST1 Polyclonal antibody

Size: 20ul / 150ul The MIST1 (29853-1-AP) by Proteintech is a Polyclonal antibody targeting MIST1 in WB, ELISA applications with reactivity to Human, Mouse, Rat samples 29853-1-AP targets MIST1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: mouse pancreas tissue, rat pancreas tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Muscle intestine stomach expression 1 (MIST1), also named BHLHA15, is a secretory cell transcription factor that belongs to the basic helix-loop-helix superfamily. MIST1 is mainly expressed in serous secreted cells and essential for the development and maturation of secretory cells. Secretory cells lacking MIST1 exhibit structural and functional defects, including alterations in cytoskeletal organization and cell polarity and improper cell-cell junction. In healthy pancreata, MIST1 facilitates cell-to-cell communication, promotes acinar cell polarity, controls Ca2+ flux, promotes unfolded protein response (UPR) homeostasis, aids in vesicle assembly, and is required for regulated exocytosis. MIST1 was downregulated in pancreatic cancer tissues compared with normal or adjacent pancreatic tissues and lower MIST1 expression in pancreatic cancer cell lines than normal acinar cells (PMID:29859299). Specification Tested Reactivity: Human, Mouse, Rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32096 Product name: Recombinant human BHLHA15 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-189 aa of NM_177455 Sequence: MKTKNRPPRRRAPVQDTEATPGEGTPDGSLPNPGPEPAKGLRSRPARAAARAPGEGRRRRPGPSGPGGRRDSSIQRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLPGLEGPGPKLYQHYQQQQQVAGGALGATEAQPQGHLQRYSTQIHSFREGT* Predict reactive species Full Name: basic helix-loop-helix family, member a15 Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 20-30 kDa GenBank Accession Number: NM_177455 Gene Symbol: MIST1 Gene ID (NCBI): 168620 RRID: AB_2935483 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7RTS1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.