Proteintech, 29895-1-AP, HMGA1 Polyclonal antibody

Size: 20ul / 150ul The HMGA1 (29895-1-AP) by Proteintech is a Polyclonal antibody targeting HMGA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 29895-1-AP targets HMGA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, MKN-45 cells Positive IHC detected in: human prostate cancer tissue, human pancreas cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HUVEC cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information HMGA1, the high mobility group A1, encodes an oncofoetal protein that remodels the chromatin structure and promotes the interaction between transcriptional regulatory proteins and DNA in different cancers (PMID:8414980, PMID:11406267). The isoforms HMGA1a and HMGA1b can cross link DNA fibers in vitro and induce chromatin clustering in vivo (PMID:21596776). Mice lacking variant of HMGA1 are diabetic, show a cardiac hypertrophy and low levels of the insulin receptor (PMID:19664187). HMGA1 protein is mainly located in nucleus, especially in heterochromatin. HMGA1 is up-regulated in many tumours, including epithelial and mesenchymal tissue-originated tumours (PMID:30614613). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31866 Product name: Recombinant human HMGA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-77 aa of BC071864 Sequence: SESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTT Predict reactive species Full Name: high mobility group AT-hook 1 Observed Molecular Weight: 20 kDa GenBank Accession Number: BC071864 Gene Symbol: HMGA1 Gene ID (NCBI): 3159 RRID: AB_2923618 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P17096 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.