Proteintech, 30020-1-AP, DAB2IP Polyclonal antibody

Size: 20ul / 150ul The DAB2IP (30020-1-AP) by Proteintech is a Polyclonal antibody targeting DAB2IP in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 30020-1-AP targets DAB2IP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HepG2 cells, rat brain tissue Positive IP detected in: 4T1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Disabled homolog 2-interacting protein (DAB2IP) is also named as ASK-interacting protein 1 (AIP-1). DAB2IP, is a new member of the Ras GTPase-activating protein family. It interacts directly with DAB2 and has distinct cellular functions such as modulating different signal cascades associated with cell proliferation, survival, apoptosis and metastasis. The aberrant alteration of DAB2IP in cancer is caused by a variety of mechanisms, including the aberrant promoter methylation, histone deacetylation, and others (PMID: 26658103). DAB2IP is reported to bind with Apoptosis signal-regulating kinase 1 (ASK1), and is called ASK1 Interacting Protein (AIP1) (PMID: 12813029). DAB2IP function is that of a tumor suppressor in tumor cell growth, metastasis, and other aspects in cancer progression. In prostate cancer, DAB2IP expression was repressed by promoter methylation and histone modification (PMID: 12446720, PMID: 15817459). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag31340 Product name: Recombinant human DAB2IP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 720-931 aa of BC146762 Sequence: RGLGDSGSEGHSSLSSHSNSEELAAAAKLGSFSTAAEELARRPGELARRQMSLTEKGGQPTVPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPNLLSTLQYPRPSSGTLASASPDWVGPSTRLRQQSSSSKGDSPELKPRAVHKQGPSPVSPNALDRTAAWLLTMNAQLLEDEGLGPDPPHRDRLRSKDELSQAEKDLAVLQDKLRISTKK Predict reactive species Full Name: DAB2 interacting protein Calculated Molecular Weight: 1189 aa, 132 kDa Observed Molecular Weight: 125-132 kDa GenBank Accession Number: BC146762 Gene Symbol: DAB2IP Gene ID (NCBI): 153090 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5VWQ8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.