Proteintech, 30071-1-AP, CUL2 Polyclonal antibody

Size: 20ul / 150ul The CUL2 (30071-1-AP) by Proteintech is a Polyclonal antibody targeting CUL2 in WB, ELISA applications with reactivity to Human, mouse samples 30071-1-AP targets CUL2 in WB, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: PC-3 cells, Raji cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Background Information The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32331 Product name: Recombinant human CUL2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 590-745 aa of BC009591 Sequence: NSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA Predict reactive species Full Name: cullin 2 Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 80-87 kDa GenBank Accession Number: BC009591 Gene Symbol: CUL2 Gene ID (NCBI): 8453 RRID: AB_3086224 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13617 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.