Proteintech, 30106-1-AP, POGZ Polyclonal antibody

Size: 20ul / 150ul The POGZ (30106-1-AP) by Proteintech is a Polyclonal antibody targeting POGZ in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to Human, mouse samples 30106-1-AP targets POGZ in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, U-251 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information POGZ, also known as MRD37, is a zinc-finger protein containing 9 C2H2 zinc fingers, a CENP-B (centromere protein-B) domain, and a DDE domain found in the bacterial transposase/retroviral integrase family. POGZ plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. POGZ plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (PMID:20562864). Heterozygous mutation of POGZ causes White-Sutton syndrome (PMID: 26739615). Expect a band approximately 155 kDa in size corresponding to POGZ by western blotting. Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag32435 Product name: Recombinant human POGZ protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1178-1400 aa of NM_015100 Sequence: GQMDQPANMPDSILLEAKESGYSDDEIMELWSTRVWQKHTACQRSKGMLVMDCHRTHLSEEVLAMLSASSTLPAVVPAGCSSKIQPLDVCIKRTVKNFLHKKWKEQAREMADTACDSDVLLQLVLVWLGEVLGVIGDCPELVQRSFLVASVLPGPDGNINSPTRNADMQEELIASLEEQLKLSGEHSESSTPRPRSSPEETIEPESLHQLFEGESETESFYGFEEADLDLMEI Predict reactive species Full Name: pogo transposable element with ZNF domain Calculated Molecular Weight: 155kd Observed Molecular Weight: 155 kDa GenBank Accession Number: NM_015100 Gene Symbol: POGZ Gene ID (NCBI): 23126 RRID: AB_2935514 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7Z3K3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.