Proteintech, 30139-1-AP, EGFR Polyclonal antibody

Size: 20ul / 150ul The EGFR (30139-1-AP) by Proteintech is a Polyclonal antibody targeting EGFR in WB, IF/ICC, FC, IP, ELISA applications with reactivity to human samples 30139-1-AP targets EGFR in WB, IF/ICC, FC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: A431 cells, HaCaT cells Positive FC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 134kd Observed Molecular Weight: 145-165 kDa GenBank Accession Number: NM_005228.5 Gene Symbol: EGFR Gene ID (NCBI): 1956 RRID: AB_3086243 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.