Proteintech, 30198-1-AP, IL-5 Polyclonal antibody

Size: 20ul / 150ul The IL-5 (30198-1-AP) by Proteintech is a Polyclonal antibody targeting IL-5 in WB, ELISA applications with reactivity to mouse samples 30198-1-AP targets IL-5 in WB, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: PMA, Ionomycin and Brefeldin A treated EL-4 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Interleukin-5 (IL-5) is a critical cytokine that regulates the growth, activation, and survival of eosinophils. IL-5 is produced by both hematopoietic and non-hematopoietic cells including T cells, granulocytes, and natural helper cells. IL-5 exerts its effects for proliferation and differentiation via receptors that comprise an IL-5-specific α and common β-subunit. Overexpression of IL-5 in vivo significantly increases eosinophils and B cells in number, while mice lacking a functional gene for IL-5 or IL-5 receptor display a number of developmental and functional impairments in B cells and eosinophil lineages. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: interleukin 5 Calculated Molecular Weight: 15 aa Observed Molecular Weight: 18-20 kDa GenBank Accession Number: NM_010558 Gene Symbol: Il5 Gene ID (NCBI): 16191 RRID: AB_3669705 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04401 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.