Proteintech, 30199-1-AP, IL-2 Polyclonal antibody

Size: 20ul / 150ul The IL-2 (30199-1-AP) by Proteintech is a Polyclonal antibody targeting IL-2 in FC (Intra), ELISA applications with reactivity to rat samples 30199-1-AP targets IL-2 in FC (Intra), ELISA applications and shows reactivity with rat samples. Tested Applications Positive FC (Intra) detected in: PMA and ionomycin treated rat splenocytes cells Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells. Specification Tested Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg0260 Product name: Recombinant Rat IL-2 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 21-155 aa of NM_053836 Sequence: MAPTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ Predict reactive species Full Name: interleukin 2 Calculated Molecular Weight: 18 kDa GenBank Accession Number: NM_053836 Gene Symbol: Il2 Gene ID (NCBI): 116562 RRID: AB_3086260 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P17108 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.