Proteintech, 30293-1-AP, IFN-gamma Polyclonal antibody

Size: 20ul / 150ul The IFN-gamma (30293-1-AP) by Proteintech is a Polyclonal antibody targeting IFN-gamma in IHC, FC (Intra), ELISA applications with reactivity to rat samples 30293-1-AP targets IFN-gamma in IHC, FC (Intra), ELISA applications and shows reactivity with rat samples. Tested Applications Positive IHC detected in: rat spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: PMA and ionomycin treated rat splenocytes cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Specification Tested Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: interferon gamma Calculated Molecular Weight: 18 kDa GenBank Accession Number: NM_138880 Gene Symbol: IFN-gamma Gene ID (NCBI): 25712 RRID: AB_3669709 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01581 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.