Proteintech, 30337-1-AP, Transferrin/TF Polyclonal antibody

Size: 150ul The Transferrin/TF (30337-1-AP) by Proteintech is a Polyclonal antibody targeting Transferrin/TF in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 30337-1-AP targets Transferrin/TF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human urine sample, HepG2 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Eg0114 Product name: Recombinant Human Transferrin/TF protein (Myc Tag, His Tag) Source: mammalian cells -derived, pHZ-KIsec Tag: Myc & 6*His Domain: 20-698 aa of BC059367 Sequence: VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKNGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCQEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP Predict reactive species Full Name: transferrin Calculated Molecular Weight: 698 aa, 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: BC059367 Gene Symbol: TF Gene ID (NCBI): 7018 RRID: AB_3669712 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P02787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.