Proteintech, 30356-1-AP, FCGR3A/CD16a Polyclonal antibody

Size: 20ul / 150ul The FCGR3A/CD16a (30356-1-AP) by Proteintech is a Polyclonal antibody targeting FCGR3A/CD16a in WB, IF-P, ELISA applications with reactivity to human samples 30356-1-AP targets FCGR3A/CD16a in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, Jurkat cells Positive IF-P detected in: mouse spleen tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1281 Product name: Recombinant human FCGR3A protein Source: e coli. -derived, Unknow Tag: N/A Domain: 80cn of BC036723 Sequence: Predict reactive species Full Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a) Calculated Molecular Weight: 29 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC036723 Gene Symbol: CD16a Gene ID (NCBI): 2214 ENSEMBL Gene ID: ENSG00000203747 RRID: AB_3086300 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.